Publications | Brown Lab

2023

Structural specializations of the sperm tail
Leung MR, Zeng J, Wang X, Roelofs MC, Huang W, Chiozzi RZ, Hevler JF, Heck AJR, Dutcher SK, Brown A, Zhang R, Zeev-Ben-Mordehai Z
Cell; https://doi.org/10.1016/j.cell.2023.05.026

Axonemal structures reveal mechanoregulatory and disease mechanisms
Walton T, Gui M, Velkova S, Fassad MR, Hirst RA, Haarman E, O'Callaghan C, Bottier M, Burgoyne T, Mitchison HM, Brown A
Nature; https://doi.org/10.1038/s41586-023-06140-2

Automated model building and protein identification in cryo-EM maps
Jamali K, Kall L, Zhang R, Brown A, Kimanius D, Scheres S
BioRxiv; https://doi.org/10.1101/2023.05.16.541002

Ciliary radial spokes
Brown A, Alford LM, Yang P
The Chlamydomonas Sourcebook Volume 3: Cell Motility and Behavior, 3rd Edition

2022

Mechanism of IFT-A polymerization into trains for ciliary transport
Meleppattu S, Zhou H, Dai J, Gui M, Brown A
Cell; 185: 4986–4998

TUBB4B variants specifically impact ciliary function, causing a ciliopathic spectrum
Mechaussier S, et al
medRxiv; https://doi.org/10.1101/2022.10.19.22280748

Didemnin B and ternatin-4 inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes
Juette MF, Carelli JD, Rundlet EJ, Brown A, Shao S, Ferguson A, Wasserman MR, Holm M, Taunton J, Blanchard SC
eLife; 11: e81608

SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules
Gui M, Croft JT, Zabeo D, Acharya V, Kollman JM, Burgoyne T, Höög JL, Brown A
Proc. Natl. Acad. Sci. USA; 119(41): e2207605119

Modern and future methods for studying macromolecular assemblies in action
Brown A, Fraternali F
Curr. Opin. Struct. Biol.; 75: i102438

Structures of VWF tubules before and after concatemerization reveal a mechanism of disulfide bond exchange
Anderson JR, Li J, Springer TA, Brown A
Blood; 140(12): 1419-1430

Ciliary central apparatus structure reveals mechanisms of microtubule patterning
Gui M, Wang X, Dutcher SK, Brown A, Zhang R
Nat. Struct. Mol. Biol.; 29: 483–492

2021

Structure of CRL2^Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates
Zhou H, Zaher MS, Walter JC, Brown A
Nucleic Acids Res.; 49: 13194-13206

De novo identification of mammalian ciliary motility proteins using cryo-EM
Gui M, Farley H, Anujan P, Anderson JR, Maxwell DW, Whitchurch JB, Botsch JJ, Qui T, Meleppattu S, Singh SK, Zhang Q, Thompson J, Lucas JS, Bingle CD, Norris DP, Roy S, Brown A
Cell; 184: 5791–5806

Cryo-EM structure of cortical microtubules from human parasite Toxoplasma gondii identifies their microtubule inner proteins
Wang X, Fu Y, Beatty WL, Ma M, Brown A, Sibley LD, Zhang R
Nat. Commun.; 12: 3065

Cryo-EM structure of an activated GPCR-G protein complex in lipid nanodiscs
Zhang M, Gui M, Wang Z-F, Gorgulla C, Yu JJ, Wu H, Sun Z-Y, Klenk C, Merklinger L, Morstein L, Hagn F, Plückthun A, Brown A, Nasr ML, Wagner G
Nat. Struct. Mol. Biol.; 28: 258–267

Structure of a microtubule-bound axonemal dynein
Walton T, Wu H, Brown A.
Nat. Commun.; 12: 477

Structures of radial spokes and associated complexes important for ciliary motility
Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A.
Nat. Struct. Mol. Biol.; 28: 29–37

2020

Primary cilia: a closer look at the antenna of cells
Brown A, Zhang R.
Curr. Biol.; 30(24): PR1494-96

Structure and activation mechanism of the BBSome membrane protein trafficking complex
Singh SK, Gui M, Koh F, Yip MCJ, Brown A
eLife; 9:e53322

2019

Mechanism of ribosome stalling during translation of a poly(A) tail
Chandrasekaran V, Juszkiewicz S, Choi J, Puglisi JD, Brown A, Shao S, Ramakrishnan V, Hegde RS
Nat. Struct. Mol. Biol.; 26:1132–1140

Structure of the decorated ciliary doublet microtubule
Ma M, Stoyanova M, Rademacher G, Dutcher SK, Brown A, Zhang R
Cell; 179(4): 909-922

Structural patching fosters divergence of mitochondrial ribosomes
Petrov A, Wood EC, Bernier CR, Norris AM, Brown A, Amunts A
Mol. Biol. Evol.; 36(2):207–219

2018

Tiny crystals have big potential for determining structures of small molecules
Brown A, Clardy J
Nature; 564:348-349

Structures of translationally inactive mammalian ribosomes
Brown A, Baird MR, Yip MCJ, Murray J, Shao S
eLife; 7:e40486

Ribosomes and cryo-EM: a duet
Brown A and Shao S
Curr. Opin. Struct. Biol.; 52:1-7.

2017

Structures of the human mitochondrial ribosome in native states of assembly
Brown A, Rathore S, Kimanius D, Aibara S, Bai X-C, Rorbach J, Amunts A, Ramakrishnan V
Nat. Struct. Mol. Biol.; 24, 866–869.

Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis
Wong W, Bai X-C, Sleebs BE, Triglia T, Brown A, Thompson JK, Jackson KE, Hanssen E, Marapana DS, Fernandez IS, Ralph SA, Cowman AF, Scheres SHW, Baum J
Nat. Microbiol.; 13;2:17031.

The structure of the yeast mitochondrial ribosome
Desai N, Brown A, Amunts A, Ramakrishnan V
Science; 355(6324):528-531.

2016

Translational termination without a stop codon
James NR, Brown A, Gordiyenko Y, Ramakrishnan V
Science; 354(6318):1437-1440.

Decoding mammalian ribosome-mRNA states by translational GTPase complexes
Shao S*, Murray J*, Brown A*, Taunton J, Ramakrishnan V, Hegde RS
Cell; 167(5):1229-1240.

Ribosome-dependent activation of stringent control
Brown A*, Fernández IS*, Gordiyenko Y, Ramakrishnan V
Nature; 534(7606):277-80.

Organisation and regulation of mitochondrial protein synthesis.
Ott M, Amunts A, Brown A
Annu. Rev. Biochem.; 85:77-101.

2015

Mapping the binding site of a cross-reactive Plasmodium falciparum PfEMP1 monoclonal antibody inhibitory of ICAM-1 binding
Lennartz F, Bengtsson A, Olsen RW, Joergensen L, Brown A, Remy L, Man P, Forest E, Barfod LK, Adams Y, Higgins MK, Jensen ATR
J. Immunol.; 195(7):3273-3283.

Structural basis for stop codon recognition in eukaryotes
Brown A*, Shao S*, Murray J, Hegde RS, Ramakrishnan V
Nature; 524(7566):493-496.

Mechanistic origin of microtubule dynamic instability and its modulation by EB proteins
Zhang R, Alushin GM, Brown A, Nogales E
Cell; 162(4):849-859.

The structure of the human mitochondrial ribosome.
Amunts A*, Brown A*, Toots J, Scheres SHW, Ramakrishnan V
Science; 348(6230):95-98.

Structure and assembly pathway of the ribosome quality control complex
Shao S, Brown A, Santhanam B, Hegde RS
Mol. Cell; 57(3): 433-444.

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions
Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G
Acta Cryst. D71; 136-153.

2014

Structure of the large ribosomal subunit from human mitochondria
Brown A*, Amunts A*, Bai X-C, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science; 346(6210):718-722.

Structure of the malaria parasite 80S ribosome bound to the anti-protozoan drug emetine
Wong W*, Bai X-C*, Brown A*, Fernández IS, Hanssen E, Condron M, Tan YH, Baum J, Scheres SHW
eLife; 3:e03080.

Structure of the yeast mitochondrial large ribosomal subunit
Amunts A*, Brown A*, Bai X-C*, Llacer JL*, Hussain T, Emsley P, Long F, Murshudov G, Scheres SHW, Ramakrishnan V
Science; 343(6178):1485-1489.

The crystal structure of fibroblast growth factor 18
Brown A, Adam LE, Blundell TL
Protein Cell; 5(5):343-7.

From (+)-epigallocatechin gallate to a simplified synthetic analogue as a cytoadherence inhibitor for P. falciparum
Gemma S, Brogi S, Patil PR, Giovani S, Lamponi S, Cappelli A, Novellino E, Brown A, Higgins MK, Mustafa K, Szestak T, Craig AG, Campiani G, Butini S, Brindisi M
RCS Advances; 4, 4769-4781.

2013

Molecular mechanism of SSR128129E, an extracellularly acting, small-molecule, allosteric inhibitor of FGF receptor signalling
Herbert C, Schieborr U, Saxena K, Juraszek J, de Smet F, Alcouffe C, Bianciotto M, Saladino G, Sibrac D, Kudlinzki D, Sreeramulu S, Brown A, Rigon P, Herault J-P, Lassalle G, Blundell TL, Rousseau F, Gils A, Schymkowitz J, Tompa P, Herbert JM, Carmeliet P, Gervasio FL, Schwalbe H, Bono F
Cancer Cell; 23(4):489-501.

Cooperative heparin-mediated oligomerization of fibroblast growth factor-1 (FGF1) precedes recruitment of FGFR2 to ternary complexes.
Brown A, Robinson CJ, Gallagher JT, Blundell TL
Biophys. J.; 104(8):1720-1730.

Molecular architecture of a complex between an adhesion protein from the malaria parasite and intracellular adhesion molecule 1
Brown A, Turner L, Christoffersen S, Zhao Y, Szestak T, Craig AG, Higgins MK
J. Biol. Chem.; 288(8):5992-6003.

Structure of the trypanosome haptoglobin-hemoglobin receptor and implications for nutrient uptake and innate immunity
Higgins MK, Tkachenko O, Brown A, Reed J, Carrington M
Proc. Natl. Acad. Sci. USA; 110(5):1905-10.

A novel domain cassette identifies Plasmodium falciparum PfEMP1 proteins binding ICAM-1 and is a target of cross-reactive, adhesion-inhibitory antibodies
Bengtsson A, Joergensen L, Rask TS, Olsen RW, Andersen MA, Turner L, Theander TG, Hviid L, Higgins MK, Craig A, Brown A, Jensen AT
J. Immunol.; 190(1):240-9.

Pre-2013

Carbohydrate binding molecules in malaria pathology
Brown A, Higgins MK
Curr. Opin. Struct. Biol.; 20(5):560-6.

Structural interactomics: informatics approaches to aid the interpretation of genetic variation and the development of novel therapeutics
Lee S*, Brown A*, Pitt WR*, Higueruelo A, Bickerton GR, Gong S, Schreyer A, Tanramluk D, Blundell TL
Mol. Biosyst.; 5(12):1456-72.

Analysis of cooperativity by Isothermal Titration Calorimetry
Brown A
IJMS; 10(8):3457-77.